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KMID : 0613820060160060955
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Journal of Life Science
2006 Volume.16 No. 6 p.955 ~ p.959
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N-Acetylglucosamine Kinase is Localized to Dendritic Lipid Rafts and Caveolae of Rat Hippocampal Neurons
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Moon Il-Soo
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Abstract
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A dynamic cycle of addition and removal of O-linked N-acetylglucosamine (O-GlcNAc) at serine and threonine residues is emerging as a key regulator of nuclear and cytoplasmic protein activity. In this work, immunocytochemistry was carried out to investigate the subcellular expression of GlcNAc kinase (NAGK, EC 2.7.1.59) that catalyzes the phosphorylation of GlcNAc to GlcNAc 6-phosphate. Immunostainings of cultured rat hippocampal neurons revealed patchy or punctate distribution of NAGK. When NAGK is doublestained with caveolin-1 or flotillin, markers for caveolae and lipid rafts, respectively, NAGK was co-localized with these markers. These results indicate that most, if not all, of the NAGK immunopunctae represent caveolae and lipid rafts, and suggest NAGK’s role in these membrane microdomains.
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KEYWORD
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N-Acetylglucosamine kinase, lipid raft, hippocampal neuron, immunocytochemistry
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